Determination of flux from a saddle field fast-atom bombardment gun

Fast - atom - bombardment mass spectrometry

A detailed study of the mass spectra of peptides produced by the new technique of fast-atom bombardment is reported. Molecular weights of unmodified peptides containing up to 21 amino acids have been determined. In favourable cases, the molecular-weight determination may be made on as little as 0.1 nmol of sample. Positive-ion and negative-ion spectra are obtained with equal facility. With samp...

Fast Atom Bombardment (FAB)-MS

Structural determination of saponins extracted from starfish by fast atom bombardment collision-induced dissociation mass spectrometry.

Three saponins were extracted and isolated from starfish by reversed-phase high performance liquid chromatography (HPLC), and analyzed by fast atom bombardment mass spectrometry (FAB-MS). Their molecular weight information could be obtained by the presence of abundant [M+Na]+ ions and weak [M+H]+ ions in FAB-MS spectra. Moreover, high resolution mass measurements of their [M+Na]+ ions were perf...

Field Desorption and Fast Atom Bombardment Mass Spectrometry of Spirostanol and Furostanol Saponins from Paris polyphylla *

Natural Products, Saponins, Molecular W eight Determ ination, Sugar Sequence, Purity Control The application of field desorption and fast atom bombardment mass spectrometry for molecular weight determination and structural elucidation o f spirostanol and furostanol saponins isolated from Paris Polyphylla Sm. (Liliaceae) is reported. The capacity and limitations o f these methods for sugar seque...

FAST ATOM BOMBARDMENT MASS SPECTROMETRY (FABMS) ANALYSIS OF AN N- TERMINAL - BLOCKED PEPTIDE

FABMS analysis of T-lb peptide before and after one cycle of Edman degradation indicated an unblocked N-terminal Thr residue for this tryptic peptide. In contrast , our data showed a molecular protonated ion, MH + for T- la peptide at 655 mass units (mu) which is 42 mu higher than the MH ion of T- 1b peptide. In addition, T- la peptide was not amenable to one cycle of manual Edman degrada...